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Tom70NTD-ABHD16Acyto-mNG
(Plasmid #195159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ABHD16A-mNG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tom70NTD
  • Alt name
    Mitochondrial import receptor subunit TOM70 N-terminal domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    198
  • Mutation
    None
  • Entrez Gene
    TOMM70 (a.k.a. TOMM70A, Tom70)
  • Promoter CMV
  • Tag / Fusion Protein
    • ABHD16A cytoplasmic domain and mNeonGreen (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tom70NTD-ABHD16Acyto-mNG was a gift from Gia Voeltz (Addgene plasmid # 195159 ; http://n2t.net/addgene:195159 ; RRID:Addgene_195159)

  • For your References section:

    An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541
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