Stim1NTD-ABHD16Acyto-mNG
(Plasmid
#195158)
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Purposemammalian expression of ER localized ABHD16A tagged with mNeonGreen
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneABHD16A-mNG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStim1NTD
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Alt nameStromal interaction molecule 1 N-terminal domain
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SpeciesH. sapiens (human)
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Insert Size (bp)702
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MutationNone
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Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
- Promoter CMV
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Tag
/ Fusion Protein
- ABHD16A cytoplasmic domain and mNeonGreen (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Stim1NTD-ABHD16Acyto-mNG was a gift from Gia Voeltz (Addgene plasmid # 195158 ; http://n2t.net/addgene:195158 ; RRID:Addgene_195158) -
For your References section:
An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541