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Addgene

V5-TurboID-Mfn1E209A
(Plasmid #195156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP-Mfn1-E209A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TurboID
  • Alt name
    TurboID (BirA mutant)
  • Species
    Synthetic
  • Insert Size (bp)
    1017
  • Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
  • Promoter CMV
  • Tag / Fusion Protein
    • Mfn1-E209A (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V5-TurboID-Mfn1E209A was a gift from Gia Voeltz (Addgene plasmid # 195156 ; http://n2t.net/addgene:195156 ; RRID:Addgene_195156)

  • For your References section:

    An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541
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