pBABE flag M33
(Plasmid
#1951)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM33
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SpeciesM. musculus (mouse)
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Insert Size (bp)1560
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Entrez GeneCbx2 (a.k.a. M33, MOD2, pc)
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Tag
/ Fusion Protein
- flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
(Kingston #1220)
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE flag M33 was a gift from Robert Kingston (Addgene plasmid # 1951 ; http://n2t.net/addgene:1951 ; RRID:Addgene_1951) -
For your References section:
The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans. Levine SS, Weiss A, Erdjument-Bromage H, Shao Z, Tempst P, Kingston RE. Mol Cell Biol 2002 Sep;22(17):6070-8. 10.1128/MCB.22.17.6070-6078.2002 PubMed 12167701
Map uploaded by the depositor.
