GFP-Sharpin
(Plasmid
#195092)
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PurposeGene Overexpression of Human SHARPIN
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHARPIN
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SpeciesH. sapiens (human)
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Entrez GeneSHARPIN (a.k.a. SIPL1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Sharpin was a gift from Jukka Westermarck (Addgene plasmid # 195092 ; http://n2t.net/addgene:195092 ; RRID:Addgene_195092) -
For your References section:
SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis. Butt U, Khan MH, Pouwels J, Westermarck J. J Cell Sci. 2022 Oct 15;135(20):jcs260627. doi: 10.1242/jcs.260627. Epub 2022 Oct 13. 10.1242/jcs.260627 PubMed 36148554