BPTI-[5,55]Gly14Ile38
(Plasmid #195059)

• Purpose: Thermal stability of BPTI
• Depositing Lab: Yutaka Kuroda
• Publication: Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi: 10.1111/febs.14941. Epub 2019 Jun 17.

Backbone

• Vector backbone: pMMHa
• Backbone size w/o insert (bp): 174
• Total vector size (bp): 3660
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: bovine pancreatic trypsin inhibitor
• Alt name: BPTI
• Species: B. taurus (bovine)
• Insert Size (bp): 173
• Mutation: Changed Alanine residues at position of 14 and 38 to Gly and Ile(Isoleucine)
• Promoter: T7 promotor
• Tag / Fusion Protein:
  • His tag and trpΔLE leader sequences (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind111 (not destroyed)
• 3′ cloning site: BamH (not destroyed)
• 5′ sequencing primer: C GTA TAC GGT GGT ATC CGT GCT AAG CGT AAC
• 3′ sequencing primer: GTT ACG CTT AGC ACG GAT ACC ACC GTA TAC G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry