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Addgene

BPTI-[5,55]Gly14Ile38
(Plasmid #195059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMHa
  • Backbone size w/o insert (bp) 174
  • Total vector size (bp) 3660
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    bovine pancreatic trypsin inhibitor
  • Alt name
    BPTI
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    173
  • Mutation
    Changed Alanine residues at position of 14 and 38 to Gly and Ile(Isoleucine)
  • Promoter T7 promotor
  • Tag / Fusion Protein
    • His tag and trpΔLE leader sequences (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind111 (not destroyed)
  • 3′ cloning site BamH (not destroyed)
  • 5′ sequencing primer C GTA TAC GGT GGT ATC CGT GCT AAG CGT AAC
  • 3′ sequencing primer GTT ACG CTT AGC ACG GAT ACC ACC GTA TAC G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPTI-[5,55]Gly14Ile38 was a gift from Yutaka Kuroda (Addgene plasmid # 195059 ; http://n2t.net/addgene:195059 ; RRID:Addgene_195059)

  • For your References section:

    Hydrophobic surface residues can stabilize a protein through improved water-protein interactions. Islam MM, Kobayashi K, Kidokoro SI, Kuroda Y. FEBS J. 2019 Oct;286(20):4122-4134. doi: 10.1111/febs.14941. Epub 2019 Jun 17. 10.1111/febs.14941 PubMed 31175706
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