BPTI-21
(Plasmid #195058)

• Purpose: simplyfied amino acid sequence by alanine scanning
• Depositing Labs: Peter Kim, Yutaka Kuroda
• Publication: Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. doi: 10.1006/jmbi.2000.3622.

Backbone

• Vector backbone: pMMHa
• Backbone size w/o insert (bp): 174
• Total vector size (bp): 3660
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: bovine pancreatic trypsin inhibitor
• Alt name: BPTI-21
• Species: B. taurus (bovine)
• Insert Size (bp): 174
• Mutation: 21 amino acids are Alanine out of 58 amino acids
• Promoter: T7 promotor
• Tag / Fusion Protein:
  • His tag and trpΔLE leader sequences (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamH (not destroyed)
• 5′ sequencing primer: No
• 3′ sequencing primer: No

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry