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pBbA5a-sufABCDSE
(Plasmid #195056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sufABCDSE
  • Species
    Escherichia coli

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Laboratory of Frederic Barras (Pasteur Institute, Paris, France) as part of the IRONPLUGNPLAY collaboration.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses the E. coli SUF operon from an IPTG-inducible promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5a-sufABCDSE was a gift from Greg Bokinsky (Addgene plasmid # 195056 ; http://n2t.net/addgene:195056 ; RRID:Addgene_195056)

  • For your References section:

    Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host. D'Angelo F, Fernandez-Fueyo E, Garcia PS, Shomar H, Pelosse M, Manuel RR, Buke F, Liu S, van den Broek N, Duraffourg N, de Ram C, Pabst M, Bouveret E, Gribaldo S, Py B, Ollagnier de Choudens S, Barras F, Bokinsky G. Elife. 2022 Mar 4;11:e70936. doi: 10.7554/eLife.70936. 10.7554/eLife.70936 PubMed 35244541
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