pBbA5a-sufCDSUB
(Plasmid
#195055)
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PurposeExpresses SUF operon from B. subtilis from IPTG-inducible promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195055 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbA5a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesufCDSUB
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SpeciesBacillus subtilis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses the B. subtilis SUF operon from an IPTG-inducible promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbA5a-sufCDSUB was a gift from Greg Bokinsky (Addgene plasmid # 195055 ; http://n2t.net/addgene:195055 ; RRID:Addgene_195055) -
For your References section:
Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host. D'Angelo F, Fernandez-Fueyo E, Garcia PS, Shomar H, Pelosse M, Manuel RR, Buke F, Liu S, van den Broek N, Duraffourg N, de Ram C, Pabst M, Bouveret E, Gribaldo S, Py B, Ollagnier de Choudens S, Barras F, Bokinsky G. Elife. 2022 Mar 4;11:e70936. doi: 10.7554/eLife.70936. 10.7554/eLife.70936 PubMed 35244541