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kanMX6-ins1
(Plasmid #195038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2400
  • Total vector size (bp) 4038
  • Vector type
    yeast genomic targeting
  • Selectable markers
    Geneticin (G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KanR
  • Alt name
    kanamycin resistance cassette
  • Alt name
    encodes resistance to Geneticin (G418)
  • Species
    S. cerevisiae (budding yeast); A. gossypii
  • Insert Size (bp)
    1600
  • Tag / Fusion Protein
    • S. cerevisiae DEG1 terminator, A. gossypii TEF promoter, KanR, A. gossypii TEF terminator, S. cerevisiae DEG1 terminator

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Longtine et al., 1998

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.

For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"

The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 sequence was amplified from S. cerevisiae gDNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kanMX6-ins1 was a gift from Gloria Brar (Addgene plasmid # 195038 ; http://n2t.net/addgene:195038 ; RRID:Addgene_195038)

  • For your References section:

    Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation. Powers EN, Chan C, Doron-Mandel E, Llacsahuanga Allcca L, Kim Kim J, Jovanovic M, Brar GA. Elife. 2022 Dec 12;11:e81086. doi: 10.7554/eLife.81086. 10.7554/eLife.81086 PubMed 36503721
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