GAL4(1-147)
(Plasmid
#195029)
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PurposeThe control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneGal4-HA-HIF1α (530-652) plasmid
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Backbone manufacturerWilliam Kaelin (Addgene plasmid # 24887)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAL4 (1-147)
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Insert Size (bp)1620
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL4(1-147) was a gift from Andrew Lassar & Cheng-Hai Zhang (Addgene plasmid # 195029 ; http://n2t.net/addgene:195029 ; RRID:Addgene_195029) -
For your References section:
Creb5 establishes the competence for Prg4 expression in articular cartilage. Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB. Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. 10.1038/s42003-021-01857-0 PubMed 33712729