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Addgene

pENTR-Creb5(T59/T61A)(HA)
(Plasmid #195025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR™/SD/DTOPO
  • Backbone manufacturer
    Life Technologies, Cat#: K2420-20
  • Vector type
    Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation)
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1620
  • Mutation
    T59/T61A
  • Entrez Gene
    CREB5
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-Creb5(T59/T61A)(HA) was a gift from Andrew Lassar & Cheng-Hai Zhang (Addgene plasmid # 195025 ; http://n2t.net/addgene:195025 ; RRID:Addgene_195025)

  • For your References section:

    Creb5 establishes the competence for Prg4 expression in articular cartilage. Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB. Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. 10.1038/s42003-021-01857-0 PubMed 33712729
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