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Addgene

LentiCRISPRCreb5gRNA
(Plasmid #195021)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 52961)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Creb5 gRNA
  • gRNA/shRNA sequence
    C TGA AGC TGC ATG TTT GTC T
  • Species
    B. taurus (bovine)
  • Entrez Gene
    CREB5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRCreb5gRNA was a gift from Andrew Lassar & Cheng-Hai Zhang (Addgene plasmid # 195021 ; http://n2t.net/addgene:195021 ; RRID:Addgene_195021)

  • For your References section:

    Creb5 establishes the competence for Prg4 expression in articular cartilage. Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB. Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. 10.1038/s42003-021-01857-0 PubMed 33712729
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