pExp-RBD-CHis
(Plasmid
#195002)
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PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOP2H
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBD
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Alt nameSARS-CoV-2 protein S-protein receptor binding domain
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SpeciesSARS-CoV-2
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter T7lac
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Tag
/ Fusion Protein
- His8 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7T (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LIC cloning used 5' site NcoI (destroyed in cloning) and 3' site XhoI (not destroyed). Please visit https://doi.org/10.1101/2023.01.10.523494 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExp-RBD-CHis was a gift from Marko Hyvönen (Addgene plasmid # 195002 ; http://n2t.net/addgene:195002 ; RRID:Addgene_195002) -
For your References section:
Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. Fischer K, Lulla A, So TY, Pereyra-Gerber P, Raybould MIJ, Kohler TN, Kaminski TS, Yam-Puc JC, Hughes R, Leiß-Maier F, Brear P, Matheson NJ, Deane CM, Hyvönen M, Thaventhiran JED, Hollfelder F. bioRxiv 2023.01.10.523494 10.1101/2023.01.10.523494
