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Addgene

pPEPT1
(Plasmid #195001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195001 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pExp-Chis
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • StrepTagII-GB1 (N terminal on backbone)
    • His8-tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • StrepTagII-GB1 (N terminal on backbone)
    • His8-tag (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was generated using LIC cloning with 5' site BsaI (destroyed in cloning) and 3' site BsaI (destroyed in cloning).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPEPT1 was a gift from Marko Hyvönen (Addgene plasmid # 195001 ; http://n2t.net/addgene:195001 ; RRID:Addgene_195001)

  • For your References section:

    A recombinant approach for stapled peptide discovery yields inhibitors of the RAD51 recombinase. Pantelejevs T, Zuazua-Villar P, Koczy O, Counsell AJ, Walsh SJ, Robertson NS, Spring DR, Downs JA, Hyvonen M. Chem Sci. 2023 Nov 21;14(47):13915-13923. doi: 10.1039/d3sc03331g. eCollection 2023 Dec 6. 10.1039/d3sc03331g PubMed 38075664
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