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Addgene

pExp-His-zBasic-RBD-Avi
(Plasmid #195000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOP2A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBD
  • Alt name
    SARS-CoV-2 protein S-protein receptor binding domain
  • Species
    SARS-CoV-2
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter T7lac
  • Tags / Fusion Proteins
    • His8-Zbasic (N terminal on insert)
    • Avi-tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site XhoI (not destroyed). Please visit https://doi.org/10.1101/2023.01.10.523494 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExp-His-zBasic-RBD-Avi was a gift from Marko Hyvönen (Addgene plasmid # 195000 ; http://n2t.net/addgene:195000 ; RRID:Addgene_195000)

  • For your References section:

    Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. Fischer K, Lulla A, So TY, Pereyra-Gerber P, Raybould MIJ, Kohler TN, Kaminski TS, Yam-Puc JC, Hughes R, Leiß-Maier F, Brear P, Matheson NJ, Deane CM, Hyvönen M, Thaventhiran JED, Hollfelder F. bioRxiv 2023.01.10.523494 10.1101/2023.01.10.523494
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