pExp-His-zBasic-RBD
(Plasmid
#194999)
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PurposeProduction of SARS-CoV2 receptor binding domain in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepExp-His
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBD
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Alt nameSARS-CoV-2 protein S-protein receptor binding domain
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SpeciesSARS-CoV-2
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter T7lac
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Tag
/ Fusion Protein
- His8-Zbasic (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7T (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExp-His-zBasic-RBD was a gift from Marko Hyvönen (Addgene plasmid # 194999 ; http://n2t.net/addgene:194999 ; RRID:Addgene_194999) -
For your References section:
Multivalent bicyclic peptides are an effective antiviral modality that can potently inhibit SARS-CoV-2. Gaynor KU, Vaysburd M, Harman MAJ, Albecka A, Jeffrey P, Beswick P, Papa G, Chen L, Mallery D, McGuinness B, Van Rietschoten K, Stanway S, Brear P, Lulla A, Ciazynska K, Chang VT, Sharp J, Neary M, Box H, Herriott J, Kijak E, Tatham L, Bentley EG, Sharma P, Kirby A, Han X, Stewart JP, Owen A, Briggs JAG, Hyvonen M, Skynner MJ, James LC. Nat Commun. 2023 Jun 16;14(1):3583. doi: 10.1038/s41467-023-39158-1. 10.1038/s41467-023-39158-1 PubMed 37328472
