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pExp-xMBP-ACE2-CHis
(Plasmid #194998)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pExp-CHis
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Species
    H. sapiens (human)
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Promoter T7lac
  • Tags / Fusion Proteins
    • His8 (C terminal on insert)
    • xMBP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExp-xMBP-ACE2-CHis was a gift from Marko Hyvönen (Addgene plasmid # 194998 ; http://n2t.net/addgene:194998 ; RRID:Addgene_194998)

  • For your References section:

    Structure-Guided Chemical Optimization of Bicyclic Peptide (Bicycle) Inhibitors of Angiotensin-Converting Enzyme 2. Harman MAJ, Stanway SJ, Scott H, Demydchuk Y, Bezerra GA, Pellegrino S, Chen L, Brear P, Lulla A, Hyvonen M, Beswick PJ, Skynner MJ. J Med Chem. 2023 Jul 27;66(14):9881-9893. doi: 10.1021/acs.jmedchem.3c00710. Epub 2023 Jul 11. 10.1021/acs.jmedchem.3c00710 PubMed 37433017
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