LV TRE-GFP-TMD-DLL1-Nbox-mutant
(Plasmid
#194937)
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PurposeLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LV TRE-GFP-TMD-DLL1-Nbox-mutant was a gift from John Ngo (Addgene plasmid # 194937 ; http://n2t.net/addgene:194937 ; RRID:Addgene_194937) -
For your References section:
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Sloas DC, Tran JC, Marzilli AM, Ngo JT. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y. 10.1038/s41587-022-01638-y PubMed 36646932