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pAdeasy-CMV-AE-EF1α-R2
(Plasmid #194928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAdeasy
  • Backbone manufacturer
    HanBio
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 9200
  • Vector type
    Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    AE
  • Alt name
    Ag85B-ESAT6
  • Species
    mycobacterium tuberculosis
  • Insert Size (bp)
    1209
  • Mutation
    a 48 bp linker (Gly3ser)4 was added between the two genes
  • GenBank ID
    ene ID: 885785 Gene ID: 886209
  • Promoter CMV
  • Tag / Fusion Protein
    • Ag85B-ESAT6 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnⅠ (not destroyed)
  • 3′ cloning site PvuⅠ (not destroyed)
  • 5′ sequencing primer CTGGTACCGCCACCATGACCGCGGGCGCG
  • 3′ sequencing primer ggcaccggagcgatcgcagatcctTCTATGCGAACATCCCAGTGA TCACTGGGATGTTCGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    EEF1A1
  • Alt name
    eukaryotic translation elongation factor 1 alpha 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    546
  • GenBank ID
    NM_001402.6
  • Entrez Gene
    EEF1A1 (a.k.a. CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1)
  • Promoter EF1a
  • Tag / Fusion Protein
    • EF1a (C terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuⅠ (not destroyed)
  • 3′ cloning site AluⅠ (not destroyed)
  • 5′ sequencing primer ACGTCACTGGGATGTTCGCATAGAaggatctgcgatcgctccg
  • 3′ sequencing primer ACGGGAAGGGTGGTGGCCATGGTGGCgtaggcgccggtcacagcttg
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    R2
  • Alt name
    Rv2031-Rv2626
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    918
  • Mutation
    a 48 bp linker (Gly3ser)4 was added between the two genes
  • GenBank ID
    Gene ID: 887579 Gene ID: 888576
  • Promoter EF1a
  • Tag / Fusion Protein
    • Rv2031c-Rv2626c (C terminal on backbone)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site AluⅠ (unknown if destroyed)
  • 3′ cloning site XhoⅠ (not destroyed)
  • 5′ sequencing primer aagctgtgaccggcgcctacGCCACCATGGCCACCACCCTTCCCGT
  • 3′ sequencing primer ataaacaagttaagcttaggctcgagCTAGCTGGCGAGGGCCATGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdeasy-CMV-AE-EF1α-R2 was a gift from Limei Wang (Addgene plasmid # 194928 ; http://n2t.net/addgene:194928 ; RRID:Addgene_194928)

  • For your References section:

    Intranasal Immunization with a Recombinant Adenovirus Encoding Multi-Stage Antigens of Mycobacterium tuberculosis Preferentially Elicited CD8(+) T Cell Immunity and Conferred a Superior Protection in the Lungs of Mice than Bacillus Calmette-Guerin. Wang L, Kang J, Jiang H. Vaccines (Basel). 2024 Sep 6;12(9):1022. doi: 10.3390/vaccines12091022. 10.3390/vaccines12091022 PubMed 39340053
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