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pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR
(Plasmid #194925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-U6-sgRNA-PGK-puromycin
  • Backbone manufacturer
    Xingxu Huang
  • Backbone size (bp) 4952
  • Modifications to backbone
    The cDNAs of mRFP and a T2A linker were inserted in between the hPGK promoter and the puromycin resistance gene (PuroR)
  • Vector type
    CRISPR
  • Promoter hPGK
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5'-CCACCGGAGCTTACCATGGCCAGCTCCGAGGATG-3'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR was a gift from Hao Xu (Addgene plasmid # 194925 ; http://n2t.net/addgene:194925 ; RRID:Addgene_194925)

  • For your References section:

    Generating a New sgRNA Vector, pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR, to Improve Base Editing. Ayo TE, Xu H. J Genome Ed Regul. 2022;2:246146. doi: 10.32371/jger/246146. Epub 2022 Dec 17. 10.32371/jger/246146 PubMed 38076397
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