PB-Hyg-Dest-mCherry-PAR-6B
(Plasmid #194908)

• Purpose: To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.
• Depositing Lab: Clare Buckley
• Publication: Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.15252/embj.2022111021.

Backbone

• Vector backbone: PB/Hygro-DEST
• Backbone size w/o insert (bp): 7600
• Total vector size (bp): 9240
• Vector type: Mammalian Expression, Mouse Targeting
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PAR-6B
• Alt name: Pard6b
• Alt name: Partitioning defective 6 homolog beta
• Species: M. musculus (mouse)
• Insert Size (bp): 897
• Mutation: 39 C to G, silent mutation
• GenBank ID: BC025147.1
• Entrez Gene: Pard6b (a.k.a. Par6b)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Resource Information

• Supplemental Documents:
  • EMBO J plasmid PB-Hyg-Dest-mCherry-PAR-6B.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry