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Addgene

pPE4max-Blasticidin
(Plasmid #194905)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194905 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV-PEmax-P2A-hMLH1dn
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 12117
  • Total vector size (bp) 12576
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BSD
  • Alt name
    Blasticidin S deaminase
  • Species
    Synthetic
  • Insert Size (bp)
    396
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cctgcagctggccaacctgcccgaccttta
  • 3′ sequencing primer aaggcacagtcgaggctgatcagcgggttt
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPE4max-Blasticidin was a gift from George Church (Addgene plasmid # 194905 ; http://n2t.net/addgene:194905 ; RRID:Addgene_194905)
  • For your References section:

    Efficient modification and preparation of circular DNA for expression in cell culture. Oliynyk RT, Church GM. Commun Biol. 2022 Dec 21;5(1):1393. doi: 10.1038/s42003-022-04363-z. 10.1038/s42003-022-04363-z PubMed 36543890