pSL1423
(Plasmid #194904)

• Purpose: pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum
• Depositing Lab: Scott Lindner
• Publication: Bennink et al - PfTurboID (unpublished)

Backbone

• Vector backbone: pSLI-TGD(1463000)
• Backbone manufacturer: Addgene #85791
• Backbone size w/o insert (bp): 7260
• Total vector size (bp): 7692
• Vector type: Plasmodium falciparum
• Selectable markers: Neomycin (select with G418) ; Human DHFR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TurboID
• Species: Synthetic
• Insert Size (bp): 960
• Promoter: None. This allows a C-terminal fusion to a protein-of-interest if integrated into its genomic locus.
• Tag / Fusion Protein:
  • GFPmut2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pSL1423.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry