pAAV-Syn-DiO-eGFP-2A-mCherry_D4H
(Plasmid #194880)

• Purpose: Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization
• Depositing Lab: Peter Scheiffele
• Publication: Czernecki et al bioRxiv 2022.11.16.516849

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4930
• Total vector size (bp): 6838
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-T2A-mCherry-D4H
• Species: M. musculus (mouse)
• Insert Size (bp): 1908
• Promoter: human Synapsin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: Synapsin FW
• 3′ sequencing primer: WPRE RV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry-D4H sequence received from Sophie Martin lab, University of Geneva

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.16.516849 for bioRxiv preprint.