pAAV-GfaABC1D-eGFP-2A-mCherry_YDA
(Plasmid #194879)

• Purpose: Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization
• Depositing Lab: Peter Scheiffele
• Publication: Czernecki et al bioRxiv 2022.11.16.516849

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5090
• Total vector size (bp): 6998
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-T2A-mCherry-YDA
• Species: M. musculus (mouse)
• Insert Size (bp): 1908
• Promoter: GfaABC1D

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: GFP RV
• 3′ sequencing primer: WPRE RV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GfaABC1D promoter was cloned in to replace Synapsin promoter from pAAV-Syn-GFP-T2A-D4H construct. Complete sequence show the D4H mutations, partial sequence has the YDA mutations.

Please visit https://doi.org/10.1101/2022.11.16.516849 for bioRxiv preprint.