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Addgene

pHEE401_GV_MOD
(Plasmid #194874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHEE401E
  • Vector type
    CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    19400
  • Promoter egg cell-specific

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer atggattacaaggaccacgacggg
  • 3′ sequencing primer gaagaagaagtga
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEE401_GV_MOD was a gift from Wolfgang Busch (Addgene plasmid # 194874 ; http://n2t.net/addgene:194874 ; RRID:Addgene_194874)

  • For your References section:

    ClearDepth: a simple, robust, and low-cost method to assess root depth in soil. Ruiz Rosquete M, Gonzalez J, Wertz K, Gonzalez N, Baez M, Wang L, Zhang L, Patil S, Funaro L, Busch W. Plant J. 2025 Jan;121(1):e17177. doi: 10.1111/tpj.17177. Epub 2024 Dec 8. 10.1111/tpj.17177 PubMed 39645605
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