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GFP-RAPH1Δ536-587
(Plasmid #194865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFPC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAPH1
  • Alt name
    Lamellipodin
  • Species
    H. sapiens (human)
  • Mutation
    RAPH1 with amino acids 536-587 deleted
  • Entrez Gene
    RAPH1 (a.k.a. ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original EGFP-Lpd plasmid was kindly provided by Matthias Krause (King’s College London).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GFP-RAPH1Δ536-587 (RAPH1 aa 536-587 deleted) construct was created by inserting a custom gene block (IDT) in the EGFP-Lpd plasmid using the HindIII/KpnI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-RAPH1Δ536-587 was a gift from Guillaume Jacquemet (Addgene plasmid # 194865 ; http://n2t.net/addgene:194865 ; RRID:Addgene_194865)

  • For your References section:

    Myosin-X recruits lamellipodin to filopodia tips. Popovic A, Miihkinen M, Ghimire S, Saup R, Gronloh MLB, Ball NJ, Goult BT, Ivaska J, Jacquemet G. J Cell Sci. 2023 Mar 1;136(5):jcs260574. doi: 10.1242/jcs.260574. Epub 2023 Mar 2. 10.1242/jcs.260574 PubMed 36861887
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