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Addgene

eGFP-RAPH1-F4
(Plasmid #194863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+N-eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAPH1
  • Alt name
    Lamellipodin
  • Species
    H. sapiens (human)
  • Mutation
    amino acids 1062-1250
  • Entrez Gene
    RAPH1 (a.k.a. ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This vector was synthesized from GenScript. Briefly, the gene fragment was synthesized using gene synthesis and cloned into pcDNA3.1(+)-N-eGFP using the BamHI/XhoI sites.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP-RAPH1-F4 was a gift from Guillaume Jacquemet (Addgene plasmid # 194863 ; http://n2t.net/addgene:194863 ; RRID:Addgene_194863)

  • For your References section:

    Myosin-X recruits lamellipodin to filopodia tips. Popovic A, Miihkinen M, Ghimire S, Saup R, Gronloh MLB, Ball NJ, Goult BT, Ivaska J, Jacquemet G. J Cell Sci. 2023 Mar 1;136(5):jcs260574. doi: 10.1242/jcs.260574. Epub 2023 Mar 2. 10.1242/jcs.260574 PubMed 36861887
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