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EGFP-MYO10-FERM-ITGBD
(Plasmid #194855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFPC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYO10 S2001_F2002insA/T2009D
  • Alt name
    myosin X
  • Species
    H. sapiens (human)
  • Mutation
    MYO10 has the following mutations S2001_F2002insA/T2009D. These mutations were introduced to inhibit binding to ITGB1.
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original EGFPC1-hMyoX plasmid (Addgene plasmid # 47608) was created by Emanuel Strehler.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The EGFP-MYO10I/TGBD construct was generated by replacing the wild-type MYO10-FERM domain (boundaries 1504-2056 in MYO10) with an MYO10 FERM domain containing the required mutations (S2001_F2002insA/T2009D) using a gene block (IDT). The gene block was inserted in EGFP-MYO10 using the PvuI and XbaI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-MYO10-FERM-ITGBD was a gift from Guillaume Jacquemet (Addgene plasmid # 194855 ; http://n2t.net/addgene:194855 ; RRID:Addgene_194855)

  • For your References section:

    Myosin-X and talin modulate integrin activity at filopodia tips. Miihkinen M, Gronloh MLB, Popovic A, Vihinen H, Jokitalo E, Goult BT, Ivaska J, Jacquemet G. Cell Rep. 2021 Sep 14;36(11):109716. doi: 10.1016/j.celrep.2021.109716. 10.1016/j.celrep.2021.109716 PubMed 34525374
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