MAC-C Sars-CoV-2 ORF3a_Q57H
(Plasmid
#194821)
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PurposeSars-CoV-2 ORF3a_Q57H in MAC-C destination vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneMAC-tag-C (#108077)
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORF3a_Q57H
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Alt nameORF3a Q57H
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SpeciesSars-CoV-2
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MutationQ57H
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Entrez GeneORF3a (a.k.a. GU280_gp03)
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Tag
/ Fusion Protein
- MAC-C (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC-C Sars-CoV-2 ORF3a_Q57H was a gift from Markku Varjosalo (Addgene plasmid # 194821 ; http://n2t.net/addgene:194821 ; RRID:Addgene_194821) -
For your References section:
The comprehensive SARS-CoV-2 ‘hijackome’ knowledge base— reveals significant changes in host cell protein expression and activation by multiple SARS-CoV-2 variants. Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P, Belanov S, Auvinen P, Vartiainen M, Laine L, Österlund P, Julkkunen I, Kakkola L. 10 May 2024, Research Square 10.21203/rs.3.rs-4322477/v1