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MAC-C Sars-CoV-2 NSP2_T85I
(Plasmid #194816)

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Item Catalog # Description Quantity Price (USD)
Plasmid 194816 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    MAC-tag-C (#108077)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NSP2_T85I
  • Alt name
    NSP2 T85I
  • Species
    Sars-CoV-2
  • Mutation
    T85I
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • MAC-C (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation in +1 localization. Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-C Sars-CoV-2 NSP2_T85I was a gift from Markku Varjosalo (Addgene plasmid # 194816 ; http://n2t.net/addgene:194816 ; RRID:Addgene_194816)

  • For your References section:

    The comprehensive SARS-CoV-2 ‘hijackome’ knowledge base— reveals significant changes in host cell protein expression and activation by multiple SARS-CoV-2 variants. Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P, Belanov S, Auvinen P, Vartiainen M, Laine L, Österlund P, Julkkunen I, Kakkola L. 10 May 2024, Research Square 10.21203/rs.3.rs-4322477/v1
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