MAC-C Sars-CoV-2 NSP12_P314L
(Plasmid
#194813)
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PurposeSars-CoV-2 NSP12_P314L in MAC-C destination vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneMAC-tag-C (#108077)
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNSP12_P314L
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Alt nameNSP12_P314L
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SpeciesSars-CoV-2
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MutationP314L
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- MAC-C (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutation in +1 localization. Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC-C Sars-CoV-2 NSP12_P314L was a gift from Markku Varjosalo (Addgene plasmid # 194813 ; http://n2t.net/addgene:194813 ; RRID:Addgene_194813) -
For your References section:
The comprehensive SARS-CoV-2 ‘hijackome’ knowledge base— reveals significant changes in host cell protein expression and activation by multiple SARS-CoV-2 variants. Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P, Belanov S, Auvinen P, Vartiainen M, Laine L, Österlund P, Julkkunen I, Kakkola L. 10 May 2024, Research Square 10.21203/rs.3.rs-4322477/v1