MAC-C Sars-CoV-2 N_R203K+G204R
(Plasmid
#194809)
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PurposeSars-CoV-2 N_R203K+G204R in MAC-C destination vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneMAC-tag-C (#108077)
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN_R203K+G204R
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Alt nameNucleocapsid R203K+G204R
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SpeciesSars-CoV-2
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MutationR203K+G204R
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Entrez GeneN (a.k.a. GU280_gp10)
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Tag
/ Fusion Protein
- MAC-C (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC-C Sars-CoV-2 N_R203K+G204R was a gift from Markku Varjosalo (Addgene plasmid # 194809 ; http://n2t.net/addgene:194809 ; RRID:Addgene_194809) -
For your References section:
The comprehensive SARS-CoV-2 ‘hijackome’ knowledge base— reveals significant changes in host cell protein expression and activation by multiple SARS-CoV-2 variants. Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P, Belanov S, Auvinen P, Vartiainen M, Laine L, Österlund P, Julkkunen I, Kakkola L. 10 May 2024, Research Square 10.21203/rs.3.rs-4322477/v1