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Addgene

CasRX_Pac
(Plasmid #194803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    dCas9g1pac
  • Backbone manufacturer
    Scott Dawson UC Davis
  • Modifications to backbone
    CasRX was PCR amplified from Addgene 109049 and moved into this Giardia expression vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRX

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CasRX_Pac was a gift from Alexander Paredez (Addgene plasmid # 194803 ; http://n2t.net/addgene:194803 ; RRID:Addgene_194803)

  • For your References section:

    A cell-cycle-dependent GARP-like transcriptional repressor regulates the initiation of differentiation in Giardia lamblia. Shih HW, Alas GCM, Paredez AR. Proc Natl Acad Sci U S A. 2022 May 31;119(22):e2204402119. doi: 10.1073/pnas.2204402119. Epub 2022 May 25. 10.1073/pnas.2204402119 PubMed 35613049
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