pcDNA5/FRT/TO GFP HSPH1
(Plasmid #19480)

• Depositing Lab: Harm Kampinga
• Publication: Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HSPH1
• Alt name: HSP105
• Species: H. sapiens (human)
• Insert Size (bp): 3335
• Entrez Gene: HSPH1 (a.k.a. HSP105, HSP105A, HSP105B, NY-CO-25)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Part of the HSP110 gene family

Disclaimer:

For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. For this particular plasmid, Addgene has confirmed that the HSPH1 has 3 point mutations: D480G, A528V, and N613S. The Kampinga lab considers these SNPs and their effect on function is not known.