pHACK(Gal4)-DONR(T2A-LexAGAD)
(Plasmid
#194769)
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PurposeTo insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACGCB(2.1)-Gal4
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2A-LexAGAD
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHACK(Gal4)-DONR(T2A-LexAGAD) was a gift from Chun Han (Addgene plasmid # 194769 ; http://n2t.net/addgene:194769 ; RRID:Addgene_194769) -
For your References section:
A toolkit for converting Gal4 into LexA and Flippase transgenes in Drosophila. Karuparti S, Yeung AT, Wang B, Guicardi PF, Han C. G3 (Bethesda). 2023 Mar 9;13(3):jkad003. doi: 10.1093/g3journal/jkad003. 10.1093/g3journal/jkad003 PubMed 36617215
