huntingtin_V2095-V3138_CbioCflag
(Plasmid #194764)

• Purpose: Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
• Depositing Lab: Cheryl Arrowsmith
• Publication: Unpublished huntingtin plasmids (unpublished)

Backbone

• Vector backbone: pBacMam2-DiEx-LIC
• Total vector size (bp): 9894
• Vector type: Mammalian Expression ; Baculovirus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HTT
• Alt name: huntingtin
• Alt name: huntingtin:TOC031-A11:C246755
• Species: H. sapiens (human)
• Mutation: none
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: CMV and P10
• Tag / Fusion Protein:
  • Avi-tag (biotin acceptor peptide) and Flag-tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
• 3′ sequencing primer: pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Kazusa DNA Research Institute Gene KIEE4296 & Original Clone ID cp94296