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huntingtin_T97-M2069_NbioCflag
(Plasmid #194763)

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Item Catalog # Description Quantity Price (USD)
Plasmid 194763 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBacMam2-DiEx-LIC
  • Total vector size (bp) 12690
  • Vector type
    Mammalian Expression ; Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTT
  • Alt name
    huntingtin
  • Alt name
    huntingtin:TOC031-E10:C247752
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Promoter CMV and P10
  • Tags / Fusion Proteins
    • Avi-tag (biotin acceptor peptide) (N terminal on insert)
    • Flag-tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
  • 3′ sequencing primer pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kazusa DNA Research Institute Gene KIEE4296 & Original Clone ID cp94296

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    huntingtin_T97-M2069_NbioCflag was a gift from Cheryl Arrowsmith (Addgene plasmid # 194763 ; http://n2t.net/addgene:194763 ; RRID:Addgene_194763)
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