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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
(Plasmid #194755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-CMV-MCS-IZsGreen
  • Backbone manufacturer
    Chaoyang Li lab
  • Backbone size w/o insert (bp) 7736
  • Vector type
    Lentiviral
  • Selectable markers
    ZsGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
  • Species
    synthetic/virus
  • Insert Size (bp)
    2424
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA3.1-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS (Chaoyang Li lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS was a gift from Chaoyang Li (Addgene plasmid # 194755 ; http://n2t.net/addgene:194755 ; RRID:Addgene_194755)

  • For your References section:

    GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry. Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C. iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481. eCollection 2022 Dec 22. 10.1016/j.isci.2022.105481 PubMed 36404916
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