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pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS
(Plasmid #194751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-MCS-13_Linker-BioID2-HA-GPI-PSS
  • Backbone manufacturer
    Chaoyang Li lab
  • Backbone size (bp) 6452
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid #80899

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid of pcDNA3.1 MCS-13 x Linker-BioID2-HA-GPI-PSS was mutated by adding restriction enzyme recognition sequence for XbaI between in 1181 and 1182 site. Then the BioID2-HA fragment was deleted to generate pcDNA3.1-MCS-13 x Linker-GPI-PSS. Next, PrP (1-22)-BioID2-HA was amplified by overlap PCR. The fragments were digested with NheI and EcoRI and then ligated into plasmid of pcDNA3.1-MCS-13 x Linker-GPI-PSS, PrP (1-22)-BioID2-HA, 789 bp. 5' Cloning Site: NheI (not destroyed), 3' Cloning Site: EcoRI (not destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS was a gift from Chaoyang Li (Addgene plasmid # 194751 ; http://n2t.net/addgene:194751 ; RRID:Addgene_194751)

  • For your References section:

    GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry. Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C. iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481. eCollection 2022 Dec 22. 10.1016/j.isci.2022.105481 PubMed 36404916
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