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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
(Plasmid #194703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIX403_Capture1_APOBEC_HA_P2A_mRuby
  • Backbone size w/o insert (bp) 10863
  • Total vector size (bp) 10094
  • Modifications to backbone
    Gateway LR clonase II reaction to insert RPS2 CDS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPS2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    879
  • GenBank ID
    NC_000016.10 NM_002952.4
  • Entrez Gene
    RPS2 (a.k.a. LLREP3, S2, uS5)
  • Promoter TRE promoter, Tet ON
  • Tag / Fusion Protein
    • APOBEC1-HA-P2A-mRuby (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1 was a gift from Eugene Yeo (Addgene plasmid # 194703 ; http://n2t.net/addgene:194703 ; RRID:Addgene_194703)

  • For your References section:

    Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Brannan KW, Chaim IA, Marina RJ, Yee BA, Kofman ER, Lorenz DA, Jagannatha P, Dong KD, Madrigal AA, Underwood JG, Yeo GW. Nat Methods. 2021 May;18(5):507-519. doi: 10.1038/s41592-021-01128-0. Epub 2021 May 7. 10.1038/s41592-021-01128-0 PubMed 33963355
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