pBABE Gal4(1-94) HSF ABC Flag ph
(Plasmid #1947)

• Depositing Lab: Robert Kingston
• Publication: Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Heat Shock Factor 1
• Alt name: HSF1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: phenylalanine mutant (F418, F492, F500 A)
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Tags / Fusion Proteins:
  • flag (N terminal on insert)
  • Gal4 DBD (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI/BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Carl Wu

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Phenylalanine Mutant. (Kingston #1154)