pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
(Plasmid
#194697)
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PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTol2
- Backbone size w/o insert (bp) 12400
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCaprola_on-mEGFP
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SpeciesSynthetic
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Insert Size (bp)2376
- Promoter elavl3(HuC)
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP was a gift from Kai Johnsson (Addgene plasmid # 194697 ; http://n2t.net/addgene:194697 ; RRID:Addgene_194697) -
For your References section:
Recording physiological history of cells with chemical labeling. Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N, Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K. Science. 2024 Feb 23;383(6685):890-897. doi: 10.1126/science.adg0812. Epub 2024 Feb 22. 10.1126/science.adg0812 PubMed 38386755