pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP
(Plasmid
#194681)
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PurposeCMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaprola_01-mEGFP
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SpeciesSynthetic
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Insert Size (bp)2340
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP was a gift from Kai Johnsson (Addgene plasmid # 194681 ; http://n2t.net/addgene:194681 ; RRID:Addgene_194681) -
For your References section:
Recording physiological history of cells with chemical labeling. Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N, Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K. Science. 2024 Feb 23;383(6685):890-897. doi: 10.1126/science.adg0812. Epub 2024 Feb 22. 10.1126/science.adg0812 PubMed 38386755
