pAAV-EF1a-DIO-mGFP-MutCREB(S133A)
(Plasmid
#194642)
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PurposeExpresses the fusion of monomeric GFP and mutant CREB(S133A) in a Cre-dependent manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMammalian Conditional Gene Expression AAV Vector (Cre-On)
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMonomeric GFP and mutant CREB(S133A) fusion
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer --- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-DIO-mGFP-MutCREB(S133A) was a gift from Jun-Hyeong Cho (Addgene plasmid # 194642 ; http://n2t.net/addgene:194642 ; RRID:Addgene_194642) -
For your References section:
Neocortical synaptic engrams for remote contextual memories. Lee JH, Kim WB, Park EH, Cho JH. Nat Neurosci. 2022 Dec 23. doi: 10.1038/s41593-022-01223-1. 10.1038/s41593-022-01223-1 PubMed 36564546