pVY-18 TC-sfGFP
(Plasmid
#194632)
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Purposefluorescent protein with tetracysteine tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETDuet
- Total vector size (bp) 5894
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetracysteine-sfGFP
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Alt nameTC-sfGFP
- Promoter T7
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Tag
/ Fusion Protein
- MGCCPGCCGGA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.08.450573v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVY-18 TC-sfGFP was a gift from Allie Obermeyer (Addgene plasmid # 194632 ; http://n2t.net/addgene:194632 ; RRID:Addgene_194632) -
For your References section:
Intracellular phase separation of globular proteins facilitated by short cationic peptides. Yeong V, Wang JW, Horn JM, Obermeyer AC. Nat Commun. 2022 Dec 22;13(1):7882. doi: 10.1038/s41467-022-35529-2. 10.1038/s41467-022-35529-2 PubMed 36550144
Map uploaded by the depositor.
