pVY-17 TC-TEM1-tag3
(Plasmid
#194631)
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Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2-39
- Total vector size (bp) 3993
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR
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Alt nameTC-TEM1-tag3
- Promoter pBAD
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Tags
/ Fusion Proteins
- MGCCPGCCGGA (N terminal on insert)
- GGSKKRKKRGGSKKRKKRGGSKKRKKR (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.08.450573v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVY-17 TC-TEM1-tag3 was a gift from Allie Obermeyer (Addgene plasmid # 194631 ; http://n2t.net/addgene:194631 ; RRID:Addgene_194631) -
For your References section:
Intracellular phase separation of globular proteins facilitated by short cationic peptides. Yeong V, Wang JW, Horn JM, Obermeyer AC. Nat Commun. 2022 Dec 22;13(1):7882. doi: 10.1038/s41467-022-35529-2. 10.1038/s41467-022-35529-2 PubMed 36550144