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pVY-11 mScarletI-tag3
(Plasmid #194626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Total vector size (bp) 6075
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet-I-(GGSKKRKKR)3
  • Alt name
    mScarlet-I-tag3
  • Promoter T7
  • Tags / Fusion Proteins
    • GGSKKRKKRGGSKKRKKRGGSKKRKKR (C terminal on insert)
    • MGHHHHHGGA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVY-11 mScarletI-tag3 was a gift from Allie Obermeyer (Addgene plasmid # 194626 ; http://n2t.net/addgene:194626 ; RRID:Addgene_194626)

  • For your References section:

    Intracellular phase separation of globular proteins facilitated by short cationic peptides. Yeong V, Wang JW, Horn JM, Obermeyer AC. Nat Commun. 2022 Dec 22;13(1):7882. doi: 10.1038/s41467-022-35529-2. 10.1038/s41467-022-35529-2 PubMed 36550144
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