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Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD
(Plasmid #194608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 5994
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176
  • Alt name
    CASP-9; ICE-LAP6; Mch6; APAF-3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    945
  • Mutation
    Deletion of N-terminal CARD domain and changed Cysteine 325 to Alanine to eliminate protease activity
  • GenBank ID
    NM_015733.5
  • Entrez Gene
    Casp9 (a.k.a. APAF-3, CASP-9, Caspase-9, ICE-LAP6, Mch6)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3X Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTGGAGACGCCATCCACG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD was a gift from Hannah Rabinowich (Addgene plasmid # 194608 ; http://n2t.net/addgene:194608 ; RRID:Addgene_194608)

  • For your References section:

    A Complex between Atg7 and Caspase-9: A NOVEL MECHANISM OF CROSS-REGULATION BETWEEN AUTOPHAGY AND APOPTOSIS. Han J, Hou W, Goldstein LA, Stolz DB, Watkins SC, Rabinowich H. J Biol Chem. 2014 Mar 7;289(10):6485-6497. doi: 10.1074/jbc.M113.536854. Epub 2013 Dec 20. 10.1074/jbc.M113.536854 PubMed 24362031
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